Vitamin B1 diversity and characterization of biosynthesis genes in cassava. ↗
Mangel N, Fudge JB, Fitzpatrick TB, Gruissem W, Vanderschuren H.
Vad undersöktes?
- Hur undersöktes det?
- We analyzed cassava germplasm for accumulation of B1 vitamers.
- Vilka undersöktes?
- 41 cassava accessions
- Vilken ämnesform?
- thiamin pyrophosphate and thiamin monophosphate
- Vilken mängd användes?
- Ingen uppgift i sammanställningen
- Hur länge pågick studien?
- Ingen uppgift i sammanställningen
Studieuppgifterna återges som originalcitat. Uppgifter som saknas här kan finnas i originalutdraget.
Vad kom studien fram till?
Författarna rapporterar att halten av vitamin B1 varierade mellan accessioner av kassava, och att bladen och lagringsrötterna hade olika profiler av B1-vitamrar. I blad var de relativa nivåerna av TPP och TMP nästan lika, medan lagringsrötterna huvudsakligen innehöll TPP. De rapporterar också negativa samband mellan B1-halt i blad och uttryck av utvalda biosyntesgener.
Vad behöver resultatet vägas mot?
Underlaget omfattar endast utvalda artikelstycken; någon samlad bedömning kan inte göras.
Läs de originalstycken som sammanställningen bygger på
AbstractVitamin B1 biosynthesis de novo is regulated by duplicated genes and a conserved riboswitch in cassava. Diversity of vitamin B1 levels in cassava leaves negatively correlates with expression of selected vitamin B1 biosynthesis genes.
AbstractVitamin B1, which consists of the vitamers thiamin and its phosphorylated derivatives, is an essential micronutrient for all living organisms because it is required as a metabolic cofactor in several enzymatic reactions. Genetic diversity of vitamin B1 biosynthesis and accumulation has not been investigated in major crop species other than rice and potato. We analyzed cassava germplasm for accumulation of B1 vitamers. Vitamin B1 content in leaves and roots of 41 cassava accessions showed significant variation between accessions. HPLC analyses of B1 vitamers revealed distinct profiles in cassava leaves and storage roots, with nearly equal relative levels of thiamin pyrophosphate and thiamin monophosphate in leaves, but mostly thiamin pyrophosphate in storage roots. Unusually, the cassava genome has two genes encoding the 4-amino-2-methyl-5-hydroxymethylpyrimidine phosphate synthase, THIC (MeTHIC1 and MeTHIC2), both of which carry a riboswitch in the 3ʹ-UTR, as well as the adenylated thiazole synthase, THI1 (MeTHI1a and MeTHI1b). The THIC and THI1 genes are expressed at very low levels in storage roots compared with the accumulation of vitamin B1, indicating only limited biosynthesis de novo therein. In leaves, vitamin B1 content is negatively correlated with THIC and THI1 expression levels, suggesting post-transcriptional regulation of THIC by the riboswitch present in the 3ʹ-UTR of the THIC mRNA and regulation of THI1 by promoter activity or alternative post-transcriptional mechanisms.
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