zinc sulfate

ChEBI · EMBL-EBI ↗ · Källdatum saknas · Källans villkor

A metal sulfate compound having zinc(2+) as the counterion.

Zinc Alleviates Diabetic Muscle Atrophy via Modulation of the SIRT1/FoxO1 Autophagy Pathway Through GPR39 ↗ · 3 mars 2025 · Källans villkor

Diabetic mice, characterized by sparse and dull fur and reduced responsiveness, showed significant increases in food intake, elevated blood glucose levels, and notable decreases in body weight compared to control mice with dense and glossy fur; however, following ZnSO4 treatment, these T1D mice demonstrated gradual weight gain, reduced blood glucose levels (p < 0.05), and a slight, non‐significant decrease in food intake (p > 0.05), as illustrated in Figure 1A. At the end of the experiment, visual inspection revealed smaller hindlimb muscles in T1D mice compared to controls, but ZnSO4 treatment helped reduce this muscle atrophy, and further analysis indicated that ZnSO4 also led to significant improvements in both limb grip strength and relative grip strength, which were reduced in T1D mice, along with increases in gastrocnemius muscle weight and GMI, which were significantly lower in T1D mice compared to controls (Figure 1B). Serum zinc levels were significantly diminished in T1D mice compared to controls, while ZnSO4 supplementation restored serum zinc levels to those of the control group. Pearson correlation analysis indicated there was a positive correlation between serum zinc levels and both relative muscle strength and GMI in T1D mice (Figure 1C). Additionally, the average cross‐sectional area of gastrocnemius muscle fibres was significantly reduced in T1D mice; ZnSO4 treatment effectively increased this cross‐sectional area (Figure 1D).

Zinc Alleviates Diabetic Muscle Atrophy via Modulation of the SIRT1/FoxO1 Autophagy Pathway Through GPR39 ↗ · 3 mars 2025 · Källans villkor

All experimental procedures were conducted in accordance with national health guidelines and received approval from the Animal Welfare and Ethics Committee of Fujian Medical University (approval no. FJMU IACUC 2021‐0378, Fuzhou, China). Male C57BL/6J mice, aged 6–7 weeks, were sourced from Shanghai SLACCAS Laboratory Animal Co., Ltd. Mice were housed at 23 ± 1°C with a 12‐h light/dark cycle and given ad libitum access to water and a standard diet.

Zinc Alleviates Diabetic Muscle Atrophy via Modulation of the SIRT1/FoxO1 Autophagy Pathway Through GPR39 ↗ · 3 mars 2025 · Källans villkor

Diabetes was induced via a single intraperitoneal injection of streptozotocin (STZ, 150 mg/kg, Sigma‐Aldrich, USA) dissolved in 0.1 M citrate buffer (pH 4.5) [18]. Control mice (Ctrl) received an equivalent volume of sodium citrate buffer. Blood glucose levels were measured 72 h post‐injection using a glucose meter (Roche, Switzerland), and mice with levels above 300 mg/dL (16.7 mmol/L) were included. Diabetic mice were randomly divided into T1D (saline‐treated) or T1D + ZnSO₄ (25 mg/kg zinc sulphate‐treated) groups. Throughout the experiment, daily monitoring of food intake and water consumption occurred, while body weight, grip strength, and fasting blood glucose levels were recorded weekly.

Zinc Alleviates Diabetic Muscle Atrophy via Modulation of the SIRT1/FoxO1 Autophagy Pathway Through GPR39 ↗ · 3 mars 2025 · Källans villkor

After six weeks of treatment, mice were euthanized, and blood and skeletal muscle samples were collected. Absolute gastrocnemius muscle mass was determined as the average weight of the left and right muscles. The gastrocnemius muscle index (GMI), calculated as absolute muscle mass (mg) divided by body weight (g), was used to normalize variations in body weight, ensuring a more objective comparison of treatment effects on muscle mass.

Zinc Alleviates Diabetic Muscle Atrophy via Modulation of the SIRT1/FoxO1 Autophagy Pathway Through GPR39 ↗ · 3 mars 2025 · Källans villkor

In C2C12 myotubes subjected to high glucose (HG; 50 mM), elevated expression of muscle atrophy markers, specifically Atrogin‐1 and MuRF1, was observed.

Zinc Alleviates Diabetic Muscle Atrophy via Modulation of the SIRT1/FoxO1 Autophagy Pathway Through GPR39 ↗ · 3 mars 2025 · Källans villkor

ZnSO4 treatment effectively mitigated the increase in Atrogin‐1 and MuRF1 expression induced by HG (Figure 4B).